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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
21/02/2014 |
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Actualizado : |
05/02/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
QUAGLIOTTO, L.; AZZIZ, G.; BAJSA, N.; VAZ, P.; PÉREZ, C.; DUCAMP, F.; CADENAZZI, M.; ALTIER, N.; ARIAS, A. |
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Afiliación : |
LETICIA QUAGLIOTTO, Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; GASTÓN AZZIZ, Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; NATALIA BAJSA, Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, ¡Montevideo, Uruguay; Sección Bioquímica, Facultad de Ciencias, UdelaR, Montevideo, Uruguay; PATRICIA VAZ, Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; CARLOS PÉREZ, Departamento de Protección Vegetal, Facultad de Agronomía, Universidad de la República, Paysandú, Uruguay; FERNANDO DUCAMPO, Departamento de Protección Vegetal, Facultad de Agronomía, Universidad de la República, Paysandú, Uruguay; MÓNICA CADENAZZI, Departamento de Protección Vegetal, Facultad de Agronomía, Universidad de la República, Paysandú, Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALICIA ARIAS, Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. |
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Título : |
Three native Pseudomonas fluorescens strains tested under growth chamber and field conditions as biocontrol agents against damping-off in alfalfa. |
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Fecha de publicación : |
2009 |
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Fuente / Imprenta : |
Biological Control, October 2009, Volume 51, Issue 1, Pages 42-50. Doi: https://doi.org/10.1016/j.biocontrol.2009.05.006 |
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ISSN : |
1049-9644 |
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DOI : |
10.1016/j.biocontrol.2009.05.006 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 1 September 2008 / Accepted 22 May 2009 / Available online 28 May 2009.
Corresponding author: Leticia Quagliotto - letty@iibce.edu.uy
Funding information: This work was financially supported by the LIA-INIA 028 Project, in the frame of the "Programa de Servicios Agropecuarios 1131-OC-UR (BID-MGAP-INIA)". |
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Contenido : |
ABSTRACT.
Alfalfa (Medicago sativa) is one of the most important crops used in Uruguay for livestock feeding. Seedling diseases, particularly damping-off, are a critical factor which limits its establishment. Three native Pseudomonas fluorescens strains, UP61.2, UP143.8 and UP148.2, previously isolated from Lotus corniculatus, were evaluated to determine their efficacy as biological control agents for alfalfa seedling diseases in the field. Their compatibility with the alfalfa-Sinorhizobium meliloti symbiosis was also assessed. In growth chamber conditions seed inoculation with Pseudomonas strains did not affect different parameters of alfalfa-rhizobium symbiosis as shown by nodulation rate and shoot dry weight of plants. The presence of the commercial inoculant strains of S. meliloti did not impair colonization by the P. fluorescens and vice versa. In field trials the dynamics of rhizobial rhizospheric populations were not affected by the presence of P. fluorescens. Each P. fluorescens strain successfully colonized alfalfa roots at adequate densities for biocontrol activity. Results showed that P. fluorescens strains provided a 10-13% increase in the number of established plants relative to the control, an intermediate result compared to the fungicide treatment (24%). The alfalfa above-ground biomass was increased by 13% and 15-18% in the presence of the fungicide and P. fluorescens strains, respectively. Therefore, results from this study demonstrated that the three P. fluorescens strains provided effective control against soil-borne pathogens and suggest a potential use in the development of a commercial inoculant to be applied for the control of legume seedling diseases.
© 2009 Elsevier Inc. All rights reserved. MenosABSTRACT.
Alfalfa (Medicago sativa) is one of the most important crops used in Uruguay for livestock feeding. Seedling diseases, particularly damping-off, are a critical factor which limits its establishment. Three native Pseudomonas fluorescens strains, UP61.2, UP143.8 and UP148.2, previously isolated from Lotus corniculatus, were evaluated to determine their efficacy as biological control agents for alfalfa seedling diseases in the field. Their compatibility with the alfalfa-Sinorhizobium meliloti symbiosis was also assessed. In growth chamber conditions seed inoculation with Pseudomonas strains did not affect different parameters of alfalfa-rhizobium symbiosis as shown by nodulation rate and shoot dry weight of plants. The presence of the commercial inoculant strains of S. meliloti did not impair colonization by the P. fluorescens and vice versa. In field trials the dynamics of rhizobial rhizospheric populations were not affected by the presence of P. fluorescens. Each P. fluorescens strain successfully colonized alfalfa roots at adequate densities for biocontrol activity. Results showed that P. fluorescens strains provided a 10-13% increase in the number of established plants relative to the control, an intermediate result compared to the fungicide treatment (24%). The alfalfa above-ground biomass was increased by 13% and 15-18% in the presence of the fungicide and P. fluorescens strains, respectively. Therefore, results from this study demonstrated that the three P. flu... Presentar Todo |
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Palabras claves : |
Biological control agent; Damping-off; Field trials; Growth chamber conditions. |
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Thesagro : |
CONTROL BIOLÓGICO; MEDICAGO SATIVA; PSEUDOMONAS FLUORESCENS. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 03149naa a2200337 a 4500
001 1012817
005 2020-02-05
008 2009 bl uuuu u00u1 u #d
022 $a1049-9644
024 7 $a10.1016/j.biocontrol.2009.05.006$2DOI
100 1 $aQUAGLIOTTO, L.
245 $aThree native Pseudomonas fluorescens strains tested under growth chamber and field conditions as biocontrol agents against damping-off in alfalfa.$h[electronic resource]
260 $c2009
500 $aArticle history: Received 1 September 2008 / Accepted 22 May 2009 / Available online 28 May 2009. Corresponding author: Leticia Quagliotto - letty@iibce.edu.uy Funding information: This work was financially supported by the LIA-INIA 028 Project, in the frame of the "Programa de Servicios Agropecuarios 1131-OC-UR (BID-MGAP-INIA)".
520 $aABSTRACT. Alfalfa (Medicago sativa) is one of the most important crops used in Uruguay for livestock feeding. Seedling diseases, particularly damping-off, are a critical factor which limits its establishment. Three native Pseudomonas fluorescens strains, UP61.2, UP143.8 and UP148.2, previously isolated from Lotus corniculatus, were evaluated to determine their efficacy as biological control agents for alfalfa seedling diseases in the field. Their compatibility with the alfalfa-Sinorhizobium meliloti symbiosis was also assessed. In growth chamber conditions seed inoculation with Pseudomonas strains did not affect different parameters of alfalfa-rhizobium symbiosis as shown by nodulation rate and shoot dry weight of plants. The presence of the commercial inoculant strains of S. meliloti did not impair colonization by the P. fluorescens and vice versa. In field trials the dynamics of rhizobial rhizospheric populations were not affected by the presence of P. fluorescens. Each P. fluorescens strain successfully colonized alfalfa roots at adequate densities for biocontrol activity. Results showed that P. fluorescens strains provided a 10-13% increase in the number of established plants relative to the control, an intermediate result compared to the fungicide treatment (24%). The alfalfa above-ground biomass was increased by 13% and 15-18% in the presence of the fungicide and P. fluorescens strains, respectively. Therefore, results from this study demonstrated that the three P. fluorescens strains provided effective control against soil-borne pathogens and suggest a potential use in the development of a commercial inoculant to be applied for the control of legume seedling diseases. © 2009 Elsevier Inc. All rights reserved.
650 $aCONTROL BIOLÓGICO
650 $aMEDICAGO SATIVA
650 $aPSEUDOMONAS FLUORESCENS
653 $aBiological control agent
653 $aDamping-off
653 $aField trials
653 $aGrowth chamber conditions
700 1 $aAZZIZ, G.
700 1 $aBAJSA, N.
700 1 $aVAZ, P.
700 1 $aPÉREZ, C.
700 1 $aDUCAMP, F.
700 1 $aCADENAZZI, M.
700 1 $aALTIER, N.
700 1 $aARIAS, A.
773 $tBiological Control, October 2009, Volume 51, Issue 1, Pages 42-50. Doi: https://doi.org/10.1016/j.biocontrol.2009.05.006
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
15/11/2015 |
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Actualizado : |
31/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - 2 |
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Autor : |
OLIVERA-MUZANTE, J.; GIL, J.; VIÑOLES, C.; FIERRO, S. |
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Afiliación : |
CAROLINA VIÑOLES GIL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Reproductive outcome with GnRH inclusion at 24 or 36h following a prostaglandin F2α-based protocol for timed AI in ewes. |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
Animal Reproduction Science, 2013, v.138, no.3-4, p. 175-179. |
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ISSN : |
0378-4320 |
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DOI : |
10.1016/j.anireprosci.2013.02.013 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 2 October 2012 / Received in revised form 19 February 2013 / Accepted 21 February 2013 / Available online 28 February 2013. |
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Contenido : |
ABSTRACT.
The objective of this experiment was to study the reproductive performance obtained after a short-interval prostaglandin (PG) F2α-based protocol for timed artificial insemination (TAI) in sheep (Synchrovine®: two injections of PG 7 d apart), including a GnRH analogue at 24 or 36h after the second PG injection. The experiment involved 296 Corriedale ewes (206 multiparous and 90 nulliparous) grazing natural pastures during the breeding season (March-April; UTU " La Carolina" , Flores Uruguay, 33° S-57° W). Ewes were assigned to three treatment groups: a) Synchrovine® (Control, n=101): two injections of D-Cloprostenol 75μg, 7 d apart, b) Synchrovine®+GnRH24 (n=98): Synchrovine® plus GnRH (busereline acetate 8.4μg) 24h after the second PG injection, and c) Synchrovine®+GnRH36 (n=97): Synchrovine® plus GnRH 36h after the second PG injection. All ewes were subjected to cervical TAI (Day 0), 44 to 47h after second PG injection, with fresh extended semen pool from six rams. Reproductive performance of ewes having ovulations and ovulation rate on Day 10, estrous cycle length in ewes that returned to estrus and non-return rate to estrus up to Day 22, fertility, prolificacy and fecundity on Day 70 were analyzed. Ewes having ovulations, ovulation rate, estrous cycle length and prolificacy did not differ between groups (P>0.05). However, non-return to estrus, fertility and fecundity was decreased in Synchrovine®+GnRH24 (P<0.05) and similar between Synchrovine® and Synchrovine®+GnRH36 (P>0.05). It was concluded that the reproductive performance obtained by Synchovine® TAI protocol was impaired by GnRH at 24h and not improved by GnRH administered at 36h after the second PG injection.
© 2013 Elsevier B.V. MenosABSTRACT.
The objective of this experiment was to study the reproductive performance obtained after a short-interval prostaglandin (PG) F2α-based protocol for timed artificial insemination (TAI) in sheep (Synchrovine®: two injections of PG 7 d apart), including a GnRH analogue at 24 or 36h after the second PG injection. The experiment involved 296 Corriedale ewes (206 multiparous and 90 nulliparous) grazing natural pastures during the breeding season (March-April; UTU " La Carolina" , Flores Uruguay, 33° S-57° W). Ewes were assigned to three treatment groups: a) Synchrovine® (Control, n=101): two injections of D-Cloprostenol 75μg, 7 d apart, b) Synchrovine®+GnRH24 (n=98): Synchrovine® plus GnRH (busereline acetate 8.4μg) 24h after the second PG injection, and c) Synchrovine®+GnRH36 (n=97): Synchrovine® plus GnRH 36h after the second PG injection. All ewes were subjected to cervical TAI (Day 0), 44 to 47h after second PG injection, with fresh extended semen pool from six rams. Reproductive performance of ewes having ovulations and ovulation rate on Day 10, estrous cycle length in ewes that returned to estrus and non-return rate to estrus up to Day 22, fertility, prolificacy and fecundity on Day 70 were analyzed. Ewes having ovulations, ovulation rate, estrous cycle length and prolificacy did not differ between groups (P>0.05). However, non-return to estrus, fertility and fecundity was decreased in Synchrovine®+GnRH24 (P<0.05) and similar between Synchrovine® a... Presentar Todo |
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Palabras claves : |
Ewe; Fertility; Fixed timed artificial insemination; GnRH; Ovulation rate; Prostaglandin. |
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Thesagro : |
FERTILIDAD; OVEJA; OVINOS; OVULACION. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 02803naa a2200313 a 4500
001 1053896
005 2019-10-31
008 2013 bl uuuu u00u1 u #d
022 $a0378-4320
024 7 $a10.1016/j.anireprosci.2013.02.013$2DOI
100 1 $aOLIVERA-MUZANTE, J.
245 $aReproductive outcome with GnRH inclusion at 24 or 36h following a prostaglandin F2α-based protocol for timed AI in ewes.$h[electronic resource]
260 $c2013
500 $aArticle history: Received 2 October 2012 / Received in revised form 19 February 2013 / Accepted 21 February 2013 / Available online 28 February 2013.
520 $aABSTRACT. The objective of this experiment was to study the reproductive performance obtained after a short-interval prostaglandin (PG) F2α-based protocol for timed artificial insemination (TAI) in sheep (Synchrovine®: two injections of PG 7 d apart), including a GnRH analogue at 24 or 36h after the second PG injection. The experiment involved 296 Corriedale ewes (206 multiparous and 90 nulliparous) grazing natural pastures during the breeding season (March-April; UTU " La Carolina" , Flores Uruguay, 33° S-57° W). Ewes were assigned to three treatment groups: a) Synchrovine® (Control, n=101): two injections of D-Cloprostenol 75μg, 7 d apart, b) Synchrovine®+GnRH24 (n=98): Synchrovine® plus GnRH (busereline acetate 8.4μg) 24h after the second PG injection, and c) Synchrovine®+GnRH36 (n=97): Synchrovine® plus GnRH 36h after the second PG injection. All ewes were subjected to cervical TAI (Day 0), 44 to 47h after second PG injection, with fresh extended semen pool from six rams. Reproductive performance of ewes having ovulations and ovulation rate on Day 10, estrous cycle length in ewes that returned to estrus and non-return rate to estrus up to Day 22, fertility, prolificacy and fecundity on Day 70 were analyzed. Ewes having ovulations, ovulation rate, estrous cycle length and prolificacy did not differ between groups (P>0.05). However, non-return to estrus, fertility and fecundity was decreased in Synchrovine®+GnRH24 (P<0.05) and similar between Synchrovine® and Synchrovine®+GnRH36 (P>0.05). It was concluded that the reproductive performance obtained by Synchovine® TAI protocol was impaired by GnRH at 24h and not improved by GnRH administered at 36h after the second PG injection. © 2013 Elsevier B.V.
650 $aFERTILIDAD
650 $aOVEJA
650 $aOVINOS
650 $aOVULACION
653 $aEwe
653 $aFertility
653 $aFixed timed artificial insemination
653 $aGnRH
653 $aOvulation rate
653 $aProstaglandin
700 1 $aGIL, J.
700 1 $aVIÑOLES, C.
700 1 $aFIERRO, S.
773 $tAnimal Reproduction Science, 2013$gv.138, no.3-4, p. 175-179.
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